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Fig. 7. Immunofluorescent light microscopic localization of F-actin, tubulin and vimentin in LNER cells treated or not with 4OHT. Monolayer cultures of LNER cells were treated with (+) or without (-) 100 nM 4OHT for 24 hours prior to being fixed and stained for F-actin, tubulin and vimentin as markers of the actin, microtubule, and intermediate filament systems, respectively. Rhodamine-phalloidin was used to detect F-actin, whereas antibodies were used to detect tubulin and vimentin. (A) Cells at low magnification. (B) Cells at higher magnification using confocal microscopy. Although no differences were apparent in F-actin or tubulin staining in LNER cells treated or not treated with 4OHT, the vimentin appeared better organized in the 4OHT-treated cells, compared to cells without 4OHT. Bar, 50 µm.
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