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Fig. 8. Evidence for vimentin association with the cadherin/catenin complex. (A) Confocal microscopic localization of vimentin in L, LN and LNER cells treated or not treated with 4OHT. Monolayer cultures of parent L cells, LN cells expressing N-cadherin and LNER cells without (-) or with (+) 4OHT were fixed and stained with anti-vimentin. The vimentin in L cells, which lack a cadherin and do not aggregate, was largely perinuclear. In LN cells, which aggregate and compact, the vimentin was organized into filaments that extended throughout the cell body. In LNER cells lacking 4OHT treatment, which aggregate but do not compact, the vimentin pattern more closely resembled that in the L cells. In contrast, in LNER cells treated with 4OHT, which aggregate and compact, the vimentin pattern was closer to that seen in the LN cells. (B) Immunoblot analysis of vimentin in LNER cells with or without 4OHT. GAPDH was included as a sample loading control. (C) Immunoblot analysis of N-cadherin (N-cad) and vimentin (Vim) immunoprecipitates from LNER cells with or without 4OHT treatment. Control (Cont) was monoclonal anti-myc. Bar, 50 µm.
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