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Fig. 5. Closest-approach analysis of horned (A) and blunt (B) microtubule plus ends to a cell-plate membrane. Solid-phragmoplast-stage cells from shoot and root meristems were examined for this analysis. The bars compose histograms of the actual densities of plus ends of microtubules at different distances from the cell-plate membrane. The line is the density distribution of plus ends of randomly distributed microtubules from the cell-plate membrane. (B) There is a significant peak in the distribution of blunt ends at 30 nm. This population is much greater than the calculated random distribution (thin line) than in A, which lacks such a peak. This suggests that about 30% of blunt-ended microtubules end at ~30 nm from the cell-plate membrane.





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