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Fig. 4. Double-immunostaining and confocal analysis of permeabilized Caco-2 cells with anti-frataxin antibody. (A) In proliferating cells at day 4 of culture, frataxin localises in elongated cytoplasmic organelles identified as mitochondria by MitoTracker Red staining. (B) Differentiated cells (day 13) were smaller in size and contained a small amount of cytoplasm. Frataxin staining appears diffuse in the cytosol and co-localises only in part with MitoTracker Red signal. The arrows indicate a site of cell-cell contact clearly positive for frataxin. Magnification used is 100x.





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