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Fig. 2. Morphological changes of hMSCs following different treatments. (A) Cultures of untreated hMSCs display a characteristic fibroblastic morphology. (B) Within 6 hours of DMSO/BHA neural induction treatment about 80% of hMSCs undergo morphological changes and acquire a pseudo-neuronal shape with refractile soma and neurite-like processes. (C) A spontaneous reversion to fibroblastic shape (black arrowhead) is evident 48 hours later. (D-F) Representative photomicrographs extracted from time-lapse sequences, each corresponding to the indicated time points. (D) Time-lapse analysis of DMSO/BHA treatment inducing hMSCs to retract their cytoplasm in the perikaryal region leaving only a few cytoplasmic extensions whose tips still adhere to substrate (white arrowheads). No outgrowth of processes from the cell soma was ever observed. (E,F) The morphological rearrangements can be mimicked by incubating hMSCs with 4 mM EDTA (E) or 10 µM cytochalasin D (F). Bar, 50 µm (A-C); 20 µm (D-F).





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