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Fig. 4. Immunostaining of hMSCs. (A-G) Untreated hMSCs. (A'-D') hMSCs treated for 6 hours with DMSO/BHA. The apparent immunoreactivity of neuronal markers (A'-D') increases in parallel to the immunoreactivity of non-neuronal markers (E',F') and for the unrelated bacterial protein ß-galactosidase (G') compared to that in untreated cells (A-G). Adherent cells with fibroblastic morphology do not show any increase in immunoreactivity (arrowheads). Similarly increased immunoreactivity is also shown by hMSCs treated with cytochalasin D (H-I) and EDTA (J-K). Bar, 10 µm.





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