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Fig. 5. Actin isoform incorporation into sarcomeric assemblies in larval muscles. Using the mef2-Gal4 driver all six actins were expressed in the visceral and body wall musculature of third larval instar. (A) The incorporation patterns for each actin into the visceral muscles. The coloured panels are the merged images of the GFP channel (green and left column) and phalloidin (red and middle column). (B) Lateral views of the individual GFPactins in a single longitudinal third instar body wall muscle, with a diagram indicating the sarcomeric localization on the right (Z indicates Z-lines). Note that GFPactin88F is excluded from Z-lines. (C) The muscle ends that interdigitate with the tendon cells (not visible) within the epidermis to anchor the muscle. This end of the sarcomeric structure of a muscle is a modified and enlarged Z-line (Reedy and Beall, 1993b).





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