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Fig. 1. Cell-free interaction of Tat with {alpha}vß3 integrin. (A) Aliquots of purified human {alpha}vß3 were incubated on plastic coated with BSA, FN, GST or GST-Tat. At the end of incubation, plastic-bound proteins were extracted and analysed by western blotting with anti-ß3 antibodies. (B) Purified {alpha}vß3 was injected at (from top to bottom) 100, 50, 25, 18, 15, 10, and 5 nM for 4 minutes over a BIAcore sensorchip coated with GST-Tat. (C) {alpha}vß3 (100 nM) or the buffer alone were injected over BIAcore sensorchips coated with GST-Tat or BSA. In all the experiments the responses (in RU) were recorded as a function of time.





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