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Fig. 6. (A,B) Permanent cell lines stably expressing EGFR-related proteins (A) or IL2R-EGFR chimeras (B), whose genotypes are depicted schematically at the top of each panel, were infected with E3-13.7-positive (+) or E3-13.7-negative (-) adenoviruses. The infected cells were pulse-labeled with a mixture of [³⁵S]cysteine and [³⁵S]methionine for 30 minutes followed by a 5-hour incubation period in chase-medium. Cell lysates were immunoprecipitated with antibodies against external epitopes of EGFR (A) or IL2R (B). Immunoprecipitates were resolved by SDS-PAGE for autoradiographic detection of radiolabeled proteins. (C) Confocal images of permeabilized cells that had been infected with the E3-13.7-positive adenovirus and stained with an IL2R-specific antibody. Areas corresponding to the enlarged insets shown beneath each image are boxed in all of the panels. Bar, 10 µm. TM, transmembrane; 679-LL and 679-AA, wild-type and mutationally inactivated lysosomal sorting-signals, respectively.

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