spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Spatiotemporal replication patterns over the entire macronucleus S phase (approximately 6-8 hours). Mid optical sections of four macronuclei after pulse-chase-pulse labelling with IdU and CldU (pulse, 30 minutes; chase, 2 hours; pulse, 30 minutes) (I-IV) and of one macronucleus after pulse labelling with CldU followed by a 24-hour chase (V). Focal organisation of IdU-labelled post-replicative DNA is preserved even after a total chase of 2.5 hours (chase for 2 hours + second pulse for 30 minutes) or of 4.5 hours (chase for 4 hours + second pulse for 30 minutes; data not shown). These foci still appear as a dispersed band suggesting that mobility of post-replicative foci is strictly limited (II-IV). Focal organisation remains preserved even after amitosis (V), but foci are scattered throughout the entire volume of the macronucleus.





Right arrow Return to article