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Fig. 5. Proteins associated to replication in Stylonychia lemnae macronuclei. Immunolocalisation of the (a-d) catalytic subunit of telomerase and of (e-f) PCNA. Accumulation of telomerase and PCNA in the replication band. Mid optical sections. (a-d) Immunolocalisation of the catalytic subunit of telomerase by FISH with probes against sequences adjacent to the template region of Stylonychia lemnae telomerase RNA. (a-c) Fluorochrome channels were scanned sequentially generating 8-bit greyscale images. (a) Telomerase RNA (red), (b) To-Pro-3 (blue). (c) Overlay, with false colours assigned to each channel. (d) Overlay; detailed view of a replication band from a-c. (magnification x4). (e-l) Immunolocalisation of PCNA in (e-i) macronuclei and (j-l) one micronucleus. Fluorochrome channels were scanned sequentially generating 8-bit greyscale images. (g,h,i,l) Overlay, with false colours assigned to each channel, PCNA, red (e,j); To-Pro-3, blue (f,k). Detailed views of replication bands (h,i).





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