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Fig. 7. Shear stress induces growth of upstream focal adhesions and reduction of downstream focal adhesion area. PAECs expressing CFP-vinculin were monitored at 2-minute intervals before and after application of flow (arrow, 20 dyn/cm2). (A) Frames from such a movie, before and after 10 minutes of flow, and a ratio image of the two time points. In this ratio image, extension of focal adhesions appears red whereas loss of focal adhesion area appears blue. (B) Gain and loss of focal adhesion area are quantified by examination of a large number of focal adhesions (80 for flow, 60 for no flow controls). For each focal adhesion the area and intensity at two time points, 6-9 minutes apart were compared. Here, the average area `lost' or `gained' is presented, normalized to the area of the focal adhesion at time 0, in addition to the change in average intensity. Error bars indicate s.e.m. Bar, 5 µm.





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