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Fig. 6. (A) N-link glycosylation contributed to the mass loss. The surface integrins isolated with either B3B11 or 12G10 were either analysed directly (-) or treated (+) with PGNase F for 2 hours at 37°C. The integrins were then separated and analysed by western blotting with JB1A. The glycosylation resulted in a decrease in the molecular masses of the 12G10- and B3B11-reactive integrins to 90 kDa, indicating that the mass differences are related to N-glycosylation levels or patterns on the two integrin species.





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