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Fig. 2. Effects of BFA on the localization of ARFRP1. HeLa cells were treated with 5 µg/ml BFA for 0, 2, 5, or 10 minutes and processed for indirect immunofluorescence analysis as described under Materials and Methods. Cells were double-stained with affinity-purified anti-ARFRP1 antibodies and monoclonal antibody to either the {gamma}-adaptin subunit of the AP-1 complex or golgin-245, followed by Cy3-conjugated anti-rabbit IgG and Alexa Fluor 488-conjugated anti-mouse IgG. Bar, 20 µm.





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