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Fig. 3. Localization of Golgi proteins in cells overexpressing ARFRP1(T31N). HeLa cells expressing HA-tagged ARFRP1(T31N) (A-D; left column) were fixed and double-stained (right column) for HA and either golgin-97 (A), TGN46 (B), galactosyl transferase (GalT; C) or GGA3 (D) followed by Cy3-conjugated anti-rat IgG and Alexa Fluor 488-conjugated anti-rabbit IgG (A,B) or anti-mouse IgG (C,D). Bar, 20 µm.