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Fig. 6. Stx1 transport in cells expressing ARFRP1 mutants. HeLa cells expressing HA-tagged ARFRP1(T31N) (A,B) or ARFRP1(Q79L) (C,D) were incubated with Cy3-conjugated Stx1 at 19.5°C for 50 minutes, washed, and directly fixed (A,C) or shifted to 37°C for 60 minutes (B,D) then fixed, and stained with rat anti-HA and mouse anti-GalT antibodies followed by Alexa Fluor 488-conjugated anti-rat and Cy5-conjugated anti-mouse IgGs. Bar, 20 µm.





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