|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. Loss of PATJ expression leads to mislocalization of Occludin and ZO-3. (A) Confocal microscopic Z-sections of control (Cl 8) and PATJ KD clone 12 (Cl 12) Caco2 cells grown on filters and labeled with affinity-purified antibodies against PATJ (in red) and occludin (Occ) (in green). In PATJ KD cells, occludin is delocalized on the lateral membrane (arrows) instead of being restricted to tight junctions (arrowheads). (B) Confocal microscopic X-Y (top panels) and Z-sections (bottom panels) of Control (CT Cl 8) and PATJ KD clone (Cl 4) of Caco2 cells grown on filters and labeled with affinity-purified antibodies against ZO-3 (in red) and occludin (Occ) (in green). In PATJ KD cells, both occludin and ZO-3 are accumulated in thick spots in the lateral membrane (arrows). (C) PATJ KD cells (Cl 4) were prepared for low-temperature embedding in K4M Lowicryl resin. Occludin (left panel) and ZO-3 (right panel) are found along the lateral membrane (arrowheads) whereas ZO-3 also occasionally accumulated (arrow) in desmosome-like structures (D). ap, apical membrane; mv, microvilli; TJ, tight junctions. Bars, 10 µm (A,B); 100 nm (C).
|
