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Fig. 5. Crb3 is accumulated in a sub-apical compartment in PATJ KD cells. Confocal microscopic X-Y (top panels) and Z-sections (bottom panels) of Control (CT Cl 8) and PATJ KD clone (Cl 12) of Caco2 cells grown on filters and labeled with antibodies against SI (in red) and affinity-purified Crb3 antibodies (in blue). SI is at the apical membrane (arrowheads) in both clones whereas Crb3 is also accumulated in a dense sub-apical compartment in clone 12 (arrows). Bars, 10 µm.