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Fig. 1. Biphasic plasma membrane blebbing in adherent apoptotic cells. (A) Analysis of blebbing kinetics in adherent cells. Top: phase contrast images of biphasic blebbing in an apoptotic SW13 cell. Images in this sequence are frames taken from supplementary material Movie 2. Bar, 10 µm. Bottom: kinetics of blebbing types in relation to PS exposure in adherent cell-lines. To the left: time analyses of apoptotic features obtained from time-lapse experiments of a variety of apoptotic adherent cell-types (time across the x-axis is in minutes counting back from the onset of annexin V binding). To the right: cartoon of the kinetics of morphological changes in adherent cells undergoing apoptosis. (B) Analysis of non-adherent cells. Top: phase contrast images of a Jurkat cell undergoing apoptosis. Only one blebbing phase is observed, and blebs appear similar to late blebs in adherent cell-lines (frames taken from supplementary material Movie 3). Bar, 10 µm. Bottom: time-analyses of blebbing, as for A, using non-adherent THP-1 and Jurkat cells.

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