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Fig. 2. Chromatin relocates into surface blebs during the late phase of plasma membrane blebbing in adherent cells. (Top) Phase contrast and (bottom) fluorescence images of an apoptotic HeLa cell transiently expressing HMGB1-YFP. Arrows in zoomed frames indicate chromatin moving outwards into large surface protrusions during late blebbing. Open arrowhead indicates a late plasma membrane bleb apparently lacking chromatin. Images are taken from supplementary material Movie 4 which is presented as a colour overlay of the HMGB1-YFP (red) and phase contrast frames. Bars, 10 µm.





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