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Fig. 3. Late blebs contain cortical ER but exclude other organelles. (A) Confocal slices at 1 µm intervals through anisomycin-treated apoptotic HeLa cells stained with an anti-KDEL antibody (red) and DAPI (blue). Arrowheads indicate examples of ER membranes accumulating at the cell periphery within large surface blebs that contain chromatin (arrows). Bar, 5 µm (B) Confocal maximum projections of anisomycin-treated apoptotic HeLa cells labelled with markers for the ER (anti-calnexin), the cis-Golgi (anti-GM130), mitochondria (anti-HSP60) and lysosomes (anti-LAMP-2). In merged images, DAPI is false-coloured blue, ER markers are red and other organelle markers (GM130, HSP60 and LAMP-2) are green. Bars, 5 µm.





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