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Fig. 6. Actin and microtubule cytoskeletons contribute to the generation of late apoptotic blebs. (A) HeLa cells were induced to undergo apoptosis by anisomycin treatment (6 hours) in the absence or presence of 1 µM latrunculin A, 100 µM Y27632, or 5 µg ml-1 nocodazole. Cells were stained with anti-KDEL antibodies and DAPI, and apoptotic cells were identified by chromatin morphology. Apoptotic cells were scored for the presence of chromatin-containing, late blebs containing cortical ER. Data points represent means±s.e.m. of three experiments (*P<0.05; **P<0.01; ***P<0.001). (B) HeLa cells were treated for 6 hours with anisomycin in the absence or presence of blebbistatin (12.5 µM). Cells were fixed, DAPI stained, then scored for the presence of late blebs (top). Values are means of three experiments±s.e.m. (***P<0.001).
