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Fig. 4. Colocalization of internalized transferrin vesicles with EHD1. Human HeLa cells were transfected with GFP-EHD1 and subjected to a 10-minute pulse with labeled transferrin (transferrin 568 nm). As depicted in A, transferrin is observed in a range of peripheral and perinuclear vesicles, whereas EHD1 localizes to tubulo-vesicular membranes. The merged image shows a partial colocalization of internalized transferrin with vesicular EHD1-containing structures. The yellow box in A marks the inset depicted as B. Examples of transferrin-containing vesicular structures aligned with EHD1-containing tubular membranes are marked by white arrows. Bar, 10 µm.





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