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Fig. 1. Effects of shear stress and receptor-dependent agonists on the tyrosine phosphorylation of PECAM-1. Primary cultures of human umbilical vein endothelial cells were either maintained under static conditions, exposed to fluid shear stress (Shear; 12 dynes cm–2, 2-120 minutes) or stimulated with either solvent (CTL), VEGF (100 ng ml–1, 5-30 minutes), histamine (Hist; 1 µmol l–1, 10-30 minutes), or ionomycin (Iono; 0.1 µmol l–1, 10-30 minutes). PECAM-1 was immunoprecipitated and the tyrosine phosphorylation was assessed by western blotting. To verify equal amounts of immunoprecipitated protein the blot was reprobed with an antibody raised against PECAM-1. The western blots shown are representative of data obtained in three independent experiments and the bar graph summarizes data obtained in three independent experiments; **P<0.01, ***P<0.001 versus static conditions.





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