spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Effect of PECAM-1 downregulation on the shear-stress-induced phosphorylation of Akt, eNOS and AMPK. Primary cultures of human endothelial cells treated with either scrambled oligonucleotides or siRNA against PECAM-1 were exposed to fluid shear stress (12 dynes cm–2, 30 or 60 minutes) and the phosphorylation and expression of (A) Akt, (B) eNOS and (C) AMPK, were detected using specific antibodies. The western blots were quantified as phospho-protein per total protein and expressed relative to the control (CTL) conditions, i.e. the signal obtained in cells treated with scrambled oligonucleotides and maintained under static conditions. The bar graphs summarize the results of six independent experiments; *P<0.05, **P<0.01 versus CTL.





Right arrow Return to article