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Fig. 6. Effect of PECAM-1 downregulation on the shear stress- and bradykinin-induced activation of eNOS. Human endothelial cells were left untreated (Control), treated with scrambled oligonucleotides (Scr) or siRNA against PECAM-1 and after 48 hours either (A) exposed to fluid shear stress (12 dynes cm–2) or (B) maintained under static conditions (Static) and stimulated with bradykinin (Bk; 10 nmol l–1, 5 minutes). The graphs presented summarize the results of four independent experiments each performed in triplicate; *P<0.05, **P<0.01 versus static conditions.





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