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Fig. 7. Comparison of the shear-stress-induced phosphorylation of eNOS, Akt and AMPK in endothelial cells from wild-type and PECAM-1^–/– mice. Confluent cultures of lung endothelial cells from wild-type (WT) or PECAM-1^–/– mice were either maintained under static conditions or subjected to fluid shear stress (Shear; 12 dynes cm^–2; 30 minutes). The phosphorylation of eNOS on Ser¹¹⁷⁷, Akt on Ser⁴⁷³ and AMPK on Thr¹⁷² was determined by western blotting with specific antibodies (duplicates are shown). The western blots were quantified as phospho-protein/total protein and expressed relative to the control (CTL) conditions, i.e. the signal obtained under static conditions. The bar graph summarizes data obtained in five independent experiments; ^*P<0.05, ^**P<0.01 versus the response recorded in cells from wild-type mice.

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