Quantification of microtubule nucleation, growth and dynamics in wound-edge cells
J Cell Sci Salaycik et al.
118: 4113
JCS02531 Supplementary Material
Files in this Data Supplement:
Movie 1
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Movie1. Wound-edge
LLCPK1 cell expressing EB1-GFP (same cell as in Fig. 2E). EB1-GFP dashes can be
observed leaving the centrosome and moving to the periphery. Last frame in
movie shows phase-contrast view of cells.
Movie 2
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Movie2. Wound-edge CHO
cells that have been transiently transfected with GFP-EB1; not all cells
express the EB1-GFP. Cell in this movie is the same cell as in Fig. 2B). Notice
the EB1-GFP dashes leaving the centrosome and traveling toward the periphery.
Last frame in movie shows phase-contrast view of cells.
Movie 3
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Movie3. Wound-edge
LLCPK1 cells expressing EB1-GFP. Three wound-edge cells are shown; more EB1-GFP
dashes are observed to target sites where cells are in contact with neighboring
cells. Last frame in the movie is a phase-contrast view of the cells.
