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Fig. 1. Microtubule organization is asymmetric in wound-edge cells. (A,B) Maximum intensity projections showing the distribution of microtubules in wound-edge LLCPK1 (A) and CHO (B) cells fixed and stained with antibodies to tubulin. (C) CHO and (D) LLCPK1 cells expressing GFP-tubulin shows the asymmetric distribution of microtubules. (E,F) Microtubule distribution in wound-edge CHO cells expressing a constitutively active form of Cdc42 (L61Cdc42) (E, right cell, marked with asterisk) and uninjected control cells (E, left cell) and expressing dominant negative N19 RhoA (F, injected cell marked with asterisk). Microtubule distribution is asymmetric in control and N19 RhoA-expressing cells but not in L61 Cdc42-expressing cells. Scale bars: 10 µm.