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Fig. 2. Microtubule nucleation at the centrosome is not biased towards the wound edge. Confocal images show non-wound-edge controls (A,E) and wound-edge cells (B,F). (A-D) CHO fibroblasts and (E-H) LLCPK1 epithelial cells expressing EB1-GFP to mark the plus ends of growing microtubules; not all cells in the culture express the construct, and these cells cannot be detected in the fluorescence micrographs. The asterisk (*) marks the position from which the growing plus ends originate. Arrows indicate direction of migration into the wound. The boxed region from individual frames of the corresponding movie sequences are shown in C, D, G and H. Movies of the cells shown are in supplementary material Movies 1 and 2. Time is indicated in minutes and seconds in the upper right corner. Scale bars: 20 µm (A,B), 10 µm (E F) and 5 µm (C,D,G,H).





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