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Fig. 2. Effect of the salt bridge on cadherin-mediated cell adhesion. (a) Adhesion of N-cadherin transfectants to wild-type or mutant N-cadherin Fc fusion proteins coated at 1 µg/ml. Extension of the N-terminus by two amino acids (GG) or the mutation Glu89Ala (E89A) inhibited adhesion to wild-type N-cadherin but, when present in opposing molecules, they formed a complementary pair resulting in enhanced adhesion. The mutation Asp134Ala (D134A) prevents coordination of calcium in the junction between domains 1 and 2 and served as a negative control. (b) Adhesion between Glu89Ala and the Gly-Gly extension mutant was ablated by the mutation Asp134Ala. (c) Enhanced adhesion between the complementary pair, Gly-Gly and Glu89Ala, compared with adhesion between wild-type N-cadherin molecules over a range of concentrations of the Fc fusion proteins.





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