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Fig. 4. Effect of the prodomain. L cells expressing N-cadherin with an uncleaved prodomain were tested for adhesion to mutant or wild-type N-cadherin Fc fusion proteins. (a) L cells adhered to the Glu89Ala (E89A) mutant but not to wild-type N-cadherin. (b) Western blot demonstrating removal of the prodomain by treatment of the L cell transfectants with trypsin. (c) After treatment, the L cells adhered strongly to wild-type N-cadherin whereas adhesion to the Glu89Ala mutant was greatly diminished.





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