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Fig. 4. InsP3R3 and InsP3R2 are closely associated with the acinar lumens in both pancreatic and submandibular acinar cells. ZO-1 immunostaining (red), InsP33 immunostaining (green) are closely associated in pancreatic (A) and submandibular (B) tissue as observed in the overlay images (column 4) and in the magnified images (column 5). (C) In submandibular tissue InsP3R3 (green) and InsP3R2 (red) are also closely associated (overlay, column 4, magnified images, column 5) and apparently surround individual cells as shown by the nuclear Hoechst 33258 staining (blue). Images are projections of a series of z planes taken through the acinus. Column 1 shows the associated phase-contrast images. Scale bars: 10 µm.





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