JCS02544 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. No accumulation of GFP-WRN was observed at regions without DNA in cells. Cytoplasm (upper panels) or regions in mitotic cells without DNA (lower panels) were irradiated with both 365 nm laser (indicated as yellow spot) and 405 nm (indicated as yellow line), respectively. Pictures before and 3 minutes after irradiation are shown.

• Supplemental Figure 2 - Fig. S2. XPA-UVDE cells transfected with GFP-tagged WRN were irradiated with UVC light through a polycarbonate isopore membrane filter with pores of 3 mm in diameter with 0 J/m², 20 J/m² or 100 J/m². Cells were fixed 15 minutes after irradiation, and stained by anti-XRCC1 antibody. Distributions of XRCC1 and WRN in XPA-UVDE cells after irradiation are shown.

• Supplemental Figure 3 - Fig. S3. Accumulation of GFP-tagged mutant or deleted WRN in HeLa cells after higher dose irradiation with 365 nm laser.

• Supplemental Figure 4 - Fig. S4. Comparison of the distribution of full-length WRN and HRDC domain of WRN in HeLa cells treated with 1 mM of camptothecin and post-incubated for 6 hours.