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Fig. 7. RT-PCR examinations of effectors for K+ channel openers. RNA samples were obtained from isolated c-Kit-immunopositive cells (ICCs) or smooth muscle cells (SMCs). NTC represents `no template control'. RT-PCR was performed with six-pair primers for 45 (Kir6.1, Kir6.2, SUR1. SUR2, and c-kit) or 40 (GAPDH) cycles. Amplified products were separated on 2.5% agarose gels and analyzed by ethidium bromide staining. The numbers in the right of each gel indicate the size marker (bp). GAPDH was amplified as an index of proper amplification, and c-kit was examined in order to confirm the c-Kit-immunoreactivity under the fluorescent microscope.





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