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Fig. 5. PGI/AMF and FN do not co-internalize and interact at the cell surface. NIH-3T3 cells were co-incubated with 25 µg ml1 bPGI/AMF and 10 µg ml1 FN-FITC for 10 (A-C) or 60 (D-F) minutes at 37°C. The cells were then fixed with 3% paraformaldehyde and bPGI/AMF was revealed with Texas Red-streptavidin (A,D) and FN with anti-FN mAb and Alexa-488 conjugated secondary antibodies (B,E). Colocalization of the two appears in yellow in the merged images (C,F). Bar, 10 µm.