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Fig. 3. In the presence of antagonists OH-flutamide and bicalutamide GFP-AR shows no or little DNA-dependent immobilization. Strip-FRAP (A,C) or combined FLIP and FRAP (B,D) of GFP-AR or the non-DNA-binding mutant GFP-AR(A573D) in the presence of 10–6 M OH-flutamide (A,B) or 10–6 M bicalutamide (C,D). Experimental settings were identical to those described in Fig. 2. Lower graphs show computer simulations corresponding to the average of best fits of strip-FRAP and FLIP-FRAP models of wild-type GFP-AR (see Table 1). The absolute values of the residuals of the fit and the experimental data are plotted below the x-axis. Larger residuals in the first second of strip-FRAPs are probably due to larger variation in the beginning of the experiment, when fluorescence changes rapidly.





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