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Fig. 2. Schematic diagram illustrating the capture of a single primary gastrulating cell from zebrafish using a ConA-coated cantilever. Using an inverted optical microscope we placed the cantilever on top of a cell (A) and pressed for 1-2 seconds with a force of ~350-500 pN (B). (C) The cantilever together with the adhering cell was then withdrawn several micrometers from the surface and the cell was allowed to settle at this position for 5-15 minutes. (D) Optical image of such a captured cell (arrow) taken 50 µm above the fibronectin surface. The full length of the cantilever is 320 µm.





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