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Fig. 4. Mesendodermal progenitor cell shape and elasticity. (A) The most common cell shapes in a wild-type cell culture. (B,C) Percentage distribution in of the different cell shapes for wild-type cultures (B) and slb/wnt11 mutant cultures (C). Shading in the diagram corresponds to the inset labelling of the different cell shapes in A. The cells used for force measurement were of the two most frequent types. (D,E) Distribution of cell diameters for cells resting on glass in wild-type (D) and slb/wnt11 cultures (E). Gaussian fits give mean±width of 17.0±3.0 µm for the wild type and 18.0±3.1 µm for slb/wnt11 mutant cells. (F) Evaluation of the spring constant k (mean±s.d.) of wild-type and slb/wnt11 mutant cells (>20 cells/genotype; ≥3 curves/cell) resting on glass measured by using the AFM tip as an indentor. There is no gross difference in k between the wild type (0.46±0.21 mN/m) and slb/wnt11 mutant (0.61±0.30 mN/m) cells. SLB, slb/wnt11; WT, wild type. Bar, 10 µm.





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