QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Gem levels increase during replication stress. (A) Cyclin B (green) and Gem (red) labeling in third-instar brain lobe. Gem is abundant in late-S-, G2- and early M-phase nuclei, whose cytoplasm labels for cyclin B. This is most evident in the lamina-precursor cells (LPCs), which undergo synchronized cell cycles as they migrate (left to right) towards the lamina furrow (LF, arrow). The cells immediately to the left of the LF are in G2 phase and have high levels of Gem in the nucleus and of cyclin B in the cytoplasm. Gem is also expressed during S and G2 phase in the nuclei of neuroblast stem cells (NB) (e.g. arrowhead). (B) Dup (red) and Gem (green) are both present in the nucleus in mid-brain neuroblast stem cells during G2 phase (yellow overlap). During mitosis, stem cells have Dup and Gem distributed throughout the cell (arrow). By contrast, the smaller daughter cells have nuclear Gem (green) during S and G2 phase but little Dup (arrowhead). Cells in G1 are positive for Dup (red) but not Gem. (C) One lobe of an Mcm6³ mutant brain. Most cells with high levels of Dup (red) also have elevated levels of Gem (green; overlap is yellow), whereas other cells have only high levels of Gem (green). (D,E) HU increases levels of Dup and Gem in neuroblasts and LPCs. (D) A lower-power micrograph shows that the mid-brain NBs have greater increases in Dup and Gem than other cells in response to replication stress. (E) Higher magnification of mid-brain stem cells from D. Neuroblast stem cells (arrows) have very high levels of Dup and Gem (bright yellow), whereas surrounding cells have high levels of Gem only (green) or normal levels of either protein. Scale bar, 50 µm (A,D), 10 µm (B,C,E).

Return to article