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Fig. 4. Pkc1p is required for the maintenance of Ca²⁺-induced polarized bud growth. The zds1 ${Delta}$ (YMM187) and zds1 ${Delta}$ pkc1-834 (YMM178) strains was grown in YPD medium to early-log phase at 25°C. The cells were synchronized in G₁ with ${alpha}$ -factor and then released into YPD medium with or without 100 mM CaCl₂ at 25°C. Samples were taken at 20-minute intervals after removal of ${alpha}$ -factor and fixed with formaldehyde. (A) F-actin staining with Rhodamine-phalloidin. (B) The cumulative percentage of cells that initiated bud formation (left) or the percentage of F-actin patches localized at the incipient bud site (right; actin patches localized at the second-cycle buds were excluded) is plotted for the same samples shown in A.

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