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Fig. 5. Mutation sites in Pkc1p. (A) The pkc1-834 mutant allele carries a single nucleotide change (A2501G) that causes replacement of asparagine 834 with aspartic acid. The stt1-1 mutant allele carries a single nucleotide change (C3304T) that causes replacement of proline 1102 with serine. (B) The amino acids P1102 and N834 of Pkc1p are important for its function. pkc1{Delta} or zds1{Delta} pkc1{Delta} cells with low-copy plasmids, carrying either PKC1 (YCp50-PKC1), stt1-1 (YCp50-PKC1P1102S) or pkc1-834 (YCp50-PKC1N834D), were spotted on YPD plate with or without 300 mM CaCl2 and grown at the indicated temperatures for 2~3 days.





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