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Fig. 3. Internalised FM4-64 is rapidly transferred to vesicles containing Syb1. (A) Endocytosed FM4-64 (red) is rapidly transferred to Syb1 vesicles (green) as judged by the overlap of the two signals (yellow) after less than 1 minute. This series shows a pre-NETO cell. Note that Syb1 vesicles at the non-growing new end remain green. (B) Ratio of green and red fluorescence at the old (O) and new (N) ends pre and post NETO. In pre-NETO cells, FM4-64 uptake (red bars) is confined to the old end despite there being a bias to Syb1 vesicles (green bars) at the opposite pole. In post-NETO cells the two poles are equivalent. Error bars indicate s.e.m. Bar, 10 µm.
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