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Fig. 6. Equatorial endocytosis in fission yeast is associated with actin. (A) A dividing Crn1-GFP cell treated with FM4-64. Transient colocalisation of endocytic vesicles and actin patches is observed at the equator as at the cell poles. (B) Mitosis in a atb2-gfp cell showing the accumulation of endocytic vesicles in the region between the separating sister chromatids and the disappearance of vesicles at the poles. As the sequence focuses on the elongation of the spindle, endocytic vesicles appear out of focus. (C) Endocytosis at cell division in atb2-gfp for3 ${Delta}$ showing the continuation of polar endocytosis in this mutant. (D) Polar endocytosis continues through cytokinesis in for3 ${Delta}$ mutants. (E) Polar endocytosis continues at cell division in atb2-gfp cdc12. (F) Equatorial endocytosis is misplaced in mid1 ${Delta}$ cells. Bars, 10 µm.

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