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Fig. 7. Relocation of endocytosis from the poles to the equator requires a functional SIN. (A,B) In cdc7, sid1 and sid2 cells the site of endocytosis moves to the equator at the permissive (25°C), but not the restrictive (36°C) temperatures. Myo2 ring formation is independent of SIN function and hence, endocytosis. For sid1 and sid2 cells the images shown are merged Myo2-GFP, FM4-64 and DAPI. (C) Quantification of equatorial endocytosis in wild-type cells (WT) and SIN mutants at 25°C (closed bars) and 36°C (open bars). Bar, 10 µm.





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