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Fig. 3. Comparison of ClC-2 and NKCC1 distribution in mouse duodenum and colon. (a-c) Double immunostaining for ClC-2 (reddish) and NKCC1 (brown) in distal colon of wild-type mouse tissue. (b,c) Show higher magnification views of surface and crypt epithelium respectively. (d-f) Similar immunostaining obtained using mouse duodenum. (e,f) Higher magnification views of villus and crypt region, respectively. Arrows in e indicate villus epithelial cell-to-cell junctions. Bars, 20 µm except for d, 30 µm.