spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. The {alpha}-like-1 RPB3 domain is involved in both cytoplasmic localization and HCR contact. (A) EGFP-RPB3 full-length and relative nested deletion constructs. (B) Subcellular localization of EGFP-RPB3 and indicated deletion construct, overexpressed in cycling (GM) myogenic C2C7 cells. (C) Whole-cell extracts of NIH 3T3 mouse fibroblasts transfected with both Flag-HCR and EGFP-RPB3 deletion constructs were immunoprecipitated with anti-Flag monoclonal antibody and analysed by western blot using anti-EGFP monoclonal antibody. The asterisks indicate a heavy-chain Ig band (top) and a non-specific band (bottom); both partially cover the signal corresponding to the RPB3-a deletion mutant (lane 3).





Right arrow Return to article