The human Rothmund-Thomson syndrome gene product, RECQL4, localizes to distinct nuclear foci that coincide with proteins involved in the maintenance of genome stability
J Cell Sci Petkovic et al.
118: 4261
JCS02556 Supplementary Material
Files in this Data Supplement:
Supplemental Figure 1
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Fig. S1. RECQL4
staining does not significantly change after treatment with different DNA
damaging agents. HeLa cells were treated with 10 mM HU, 50 J/m2 UV
and 5 Gy. RECQL4 (red), DNA (blue), Bar, ~10 mm.
Supplemental Figure 2
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Fig. S2. Knockdown of RECQL4 expression inhibits the
RECQL4/Rad51 colocalization. Control and RECQL4-1 siRNA transfected HeLa cells
were treated with 10 mm etoposide followed by 12 hours recovery at 37°C. Cells were fixed,
permeabilized and stained with anti-RECQL4 (red), anti-Rad51 (green)
antibodies and DAPI (blue) as indicated.
