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Fig. 1. Characterization of anti-RecQL4 antibodies. (A) Western blot using the anti-RECQL4 N-t and anti-RECQL4 C-t against HeLa total cell extract (TCE), HeLa cytosolic extracts (CE), HeLa nuclear extract (NE), and nuclear extracts from AG18371 fibroblasts derived from a Rothmund-Thomson-syndrome patient. Control western blots against HeLa CE and HeLa NE were performed with anti-PARP1, anti-BLM and anti-GRB2 antibodies, respectively. (B) Lysates of 293T cells transiently transfected with a vector expressing FLAG-RECQL4 or with the corresponding empty vector were subjected to western blot with anti-FLAG and anti-RECQL4 C-t antibodies. Asterisks indicate breakdown products.





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