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Fig. 3. Determination of the specificity of the RECQL4 antibodies by siRNA. (A) Western blot with anti-RECQL4 C-t antibody (upper panel) or anti-tubulin antibody (lower panel) using total cell extracts from HeLa cells transfected with 200 nM RECQL4-1 or control-1 siRNA. Asterisks indicate breakdown products. (B) RECQL4 knock-down leads to a loss of RECQL4 nuclear foci. Immunofluorescence analysis with anti-RECQL4 N-t antibody (red) on exponentially growing HeLa cells transfected with 200 nM RECQL4-1 or control-1 siRNA, DAPI-staining shows nuclear DNA (blue). Bar, ~10 µm. (C) The mean number of RECQL4 foci per cell after siRNA treatment is displayed in histograms (standard error of the mean is indicated in the columns).





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